CHE 326: SCHEDULE

Biochemistry I

Spring Semester: 2004

Week 1: Essential Tools and Concepts

Suggested Reading and Goals forWeek 1

Monday, January 12^th: Introduction to the Course: Review of Chemistry Background:

Functional Macromolecules

Bonds, Blocks and Polymers

Wednesday January 14^th: Review of Cell and Molecular Biology Background:

The Basics of Bioinformatics

DNA: The Molecule

Finding and Reading a Scientific Paper
 

Friday January 16^th Other Major Molecules of Biochemistry and Molecular Biology: Carbohydrates and Lipids

Study Questions 1:

Week 2: Protein Structure: Malate Dehydrogenase as an Example 

Suggested Reading and Goals for Week 2

Monday January 19^th: :  Basic Structures and Properties of Amino Acids

Review Topic: What is a Protein?

Wednesday January 21^st: Protein Primary Structure
Precursors and Cellular Localization

Friday January 23^rd: Secondary, Tertiary and Quaternary Structure of Proteins

Study Questions 2:
 

Week 3: Purification, Characterization and Classification of Proteins

Suggested Reading and Goals forWeek 3

Monday  January 26^th:  SNOW DAY

Wednesday January 28^th :
Assays of Protein Activity

Problem Set 2 Answers

Steps in the Purification of a Protein

Trial Questions for the First Exam

Friday 30^th: :   Molecular Characterization of Proteins

Molecular Weights and Quaternary Structure

Study Questions 3:

Week 4: Domains, Folds and Functions

Suggested Reading and Goals forWeek 4

Monday  February 2^nd:First In Class Exam: Material to Wednesday January 30^th:

Answer key

Wednesday February 4^thBasics of Structure Determination by X Ray Crystallography and NMR

Friday February 6^th:   Classes of Proteins: Specialized Proteins I: Transport

Classes of Proteins: Specialized Proteins II: Structural and Mechanical

Specialized Proteins: Defense

Study Questions 4:

Week 5: Enzyme Chemistry, Catalysis and Kinetics

Suggested Reading and Goals forWeek 5

Monday February 9^th: Enzyme Chemistry : Theory

Wednesday February 11^thEnzyme Kinetics: The Basics

Friday February 13^th: Enzyme Kinetics: Continued

Study Questions 5

Week 6: Paradigms of Activity Regulation

Suggested Reading and Goals forWeek 6

Monday February 16^th:   Enzyme Kinetics, Inhibition and Regulation

Derivations of Initial Rate and Enzyme Inhibitor Equations
Wednesday February 18^th:Allosteric Regulation

Trial-Questions-for-the second-in-class-exam

Friday February 20^th:Regulation by Covalent Modification

Study Questions 6:

Week 7: Membranes and Compartmentation

Suggested Reading and Goals forWeek 7

Monday February 23^rd:Membrane Structure:Sugars and Oligosaccharides, Glycoproteins and Glycolipids
Cell Surface Receptors and Transport and Signaling Across Membranes
Fats, Lipids and Membranes

Wednesday February 25^th  What is Metabolism and Ways of Studying Metabolism

Friday February 27^th Second In Class Exam: Quantitative Questions
 

Study Questions 7:

Week 8: Metabolism: The Big Picture

Suggested Reading and Goals forWeek 8

Monday March 1^st: In Class Oral Exams:

Wednesday March 3^rd: Biological Compartmentation I: Structural, Chemical

Friday March 5^thInter-relationships in Metabolism and Key Metabolites

Study Questions 8:

March 6^th-March 14^th: Spring Break 

Week 9: Central Roles of Glucose and Acetyl Coenzyme A 

Suggested Reading and Goals forWeek 9

Monday March 15^th: Catabolism and Anabolism, Glycogen and Glucose : I
 Generation of Reducing Equivalents

Wednesday March 17th  : No CLASS: READ and Write Out Schemes for: Acetyl Co A from Glucose

Friday March 19^thFat Metabolism : I

Study Questions 9:

Week 10: Protein Metabolism

Suggested Reading and Goals forWeek 10

Monday March 22^nd:  Degradation of Proteins

Wednesday March 24^thThe Central Enzymes of Amino Acid Metabolism

Friday March 26^th:       Krebs and Urea Cycles : Amino Acids

Regulation of the Urea Cycle:

Study Questions 10:

Week 11: The Central Cycles and Oxidative Phosphorylation

Suggested Reading and Goals forWeek 11

Monday March 29^th : Urea Cycle

Wednesday March 31^st:  Krebs Cycle

Friday April 2^nd: The Many Roles of Acetyl CoA

Steroid-Cholesterol- Metabolism and Signaling

Study Questions 11:

Week 12: 

Suggested Reading and Goals forWeek 12

Monday April 5^thOxidative Phosphorylation and Photosynthesis

Wednesday  April 7^th Oxidative Phosphorylation and Photosynthesis

Friday April 9^th Hormonal Regulation and Integration of Metabolism

Study Questions 12:

Week 13:  DNA Replication and Repair  & Transcription and Regulation

Suggested Reading and Goals forWeek 13

Monday April 12^thThird In Class Exam: Material to April 9th..

Wednesday 14^th :The Central Dogma I: Replication-I

Friday April 16^th: The Central Dogma II: Transcription

Study Questions 13:

Week 14: Translation and Translocation of Proteins

Suggested Reading and Goals forWeek 14

Monday April 19^th:Elucidating the Structure of the Ribosome

Wednesday April 21^st: The Central Dogma III: Translation

Friday April 23^rd:   Packaging and Processing of Proteins

Study Questions 14